Mightex Systems developed and produced microscopy light sources that are collimated LED light sources designed for fluorescence microscopy in the research laboratory. These light sources are ready to be mounted on existing lamp ports of various microscopes to replace traditional light sources such as incandescent lamps and arc lamps.
LEDs have many desirable features for fluorescence microscopy applications:
• LED intensity can be directly modulated through driving current. This gives you accurate control over intensity, which means less bleaching and phototoxicity, and no need for neutral density filters.
• LEDs also have a fast response time, in some cases as short as 10ns. It is easy to coordinate LED illumination time with camera acquisition time, decreasing the likelihood of bleaching and phototoxicity.
• With the state-of-the-art high-brightness technology spectral density of LEDs have reached or exceeded that of some short-arc lamps. LEDs may also be pulsed at a current much higher than the normal rating, resulting in higher output (Visit the BioLED range page to see how this makes a difference).
• Another feature of LEDs is their high stability and repeatability of output intensity compared to that of short-arc lamps. For this reason LED sources are fast becoming the leading choice for quantitative measurements.
• LED light sources are also compact and dissipating less heat than many other light sources, leading to healthier samples.
• Finally, the long lifetime of LEDs translates into low cost of ownership and trouble-free operation possibly for the lifetime of the microscope.
Several single-bandwidth (up to 8 or more) LEDs can be combined and coupled into the epifluorescence port of most major microscope led models. This is done by connecting the LEDs to multi-wavelength beam combiners, using dichroic mirrors and beamsplitters to direct light from all LEDs into the epifluorescence port of the microscope.
Combining LED bands widens the options you have for the desired band of excitation light and allows you to combine LED bands to increase the overall intensity.
The mirror holders in the beamsplitters can be adjusted to optimise alignment of your optical system, and each input port can accommodate a bandpass filter to clean up the LED spectrum. This means you can eliminate cross-talk between different LED excitation bands.
The combined LED assembly can be coupled directly to the epifluorescence port of the microscope or via a light guide, to save space around your setup.
The Mightex microscope LEDs are exceptionally flexible because of their modular design. This makes your investment more future proof, since you can easily
Mightex’s MLS series of microscopy light sources also employs aspherical collimating optics to generate uniform illumination with at the sample plane. Light is also collimated in a way that optimizes light delivery, which means that you lose the minimum amount of light before it arrives at the sample level.
Mightex’s MLS series LEDs include 2-meter cables to connect to a Mightex LED controllers. A wide range of Mightex LED controller models are available, from 2 channels up to 16 channels.
Other interesting options for controller models are
Talk to us about which option would be most suitable for your application.
Please visit the Mightex Systems website to find more information about the Multi-Wavelength Collimated Microscope LEDs.