High-resolution deep tissue imaging without being highly invasive is difficult to achieve. Therefore, a new imaging method that allows non- or minimally-invasive image acquisition in vivo and in real time with sub-cellular spatial resolution, and at sub-second sampling rates is needed.
MCI now offers an exciting new imaging method that allows minimally- invasive image acquisition in vivo and in real time with sub-cellular spatial resolution – the MCI Pryer Endoscopic Objective (abbreviated as MCI Pryer). The MCI Pryer is a combined endoscopic objective and is compatible with any fluorescence microscopes, such as current 2P (2 photon) microscopes from Olympus and Nikon. This state-of-the-art objective allows for deep tissue access and visualisation in anaesthetised animals, awake animals with head fixation and even chronic recordings with our custom-built adaptor. If you are involved in 2 photo microscopy and 2 photon imaging then this endoscopic objective may well be perfect for you.
If you would like to read more about this subject then click here for our detailed introduction to the MCI Pryer Endoscopic Objective
The advantages of using MCI Pryer in a nutshell
The Pryer needle-tip of the Pryer objective is 7.85 or 5.87 mm in length, depending on which magnification objective you use. And, with a working distance of 500 or 200 µm, respectively, the Pryer objectives can image deep within the brain.
Yes, it can. In fact we highly recommend you use a cannula to enable long-term or chronic imaging.
Yes you can. The pryer objective is a great addition to your current imaging setup enabling you to image deep inside the brain and even enables you to observe activity in sub-cellular compartments, like dendrites, in real time.